Phenotype Details
Phene ID
34
Name
Deficiency of uridine monophosphate synthase
Phene Name
Haplotype HHD
OMIA ID
262
Species ID
9913
Characterised
Yes
Characterised Year
1993
Linked Genes
SymbolGene IDChromosomeDescription
UMPS2815681uridine monophosphate synthetase
Linked Variants
Variant IDPhenotypeGene IDDeleteriousChromosomeGenomicTranscriptProtein
290Deficiency of uridine monophosphate synthase28156811NC_037328.1:g.69151931C>TNM_177508.1:c.1213C>TNP_803474.1:p.(R405*)
Linked Breeds
BreedBreed IDSpecies IDVBO Term
Holstein (black and white) (Cattle)399913http://purl.obolibrary.org/obo/VBO_0000237
Holstein Friesian (Cattle)739913http://purl.obolibrary.org/obo/VBO_0000239
Red Holstein, Switzerland (Cattle)2439913http://purl.obolibrary.org/obo/VBO_0004501
Wagyu (Cattle)339913http://purl.obolibrary.org/obo/VBO_0000422
Summary

This disorder is of particular interest in cattle, because it is one of the few cases in which an embryonic lethal has been identified. (By their very nature, embryonic lethals are difficult to identify; their only manifestation is a return to service.) To date, the only known cause of this disorder in cattle is a nonsense mutation in codon 405 of the UMPS gene, resulting in a complete deficiency of functional UMPS (Schwenger et al., 1993). Since nucleotides are required in such vast quantities during embryonic development, it is not surprising that homozygosity for the nonsense mutation results in embryonic death around 40 days in utero. The practical effect of this disorder is that carrier cows show a higher rate of return to service, because some of their pregnancies end in early natural abortion. Given that returns to service can have so many different causes, it would not have been possible to identify the mutation from reproductive records. How, then, was this embryonic lethal mutation detected initially? The answer is: by chance! As part of a nutrition study at the University of Illinois, the level of orotic acid was determined in the milk of cows in the university herd. Some cows had exceptionally high levels of this acid, and one possible explanation was that they were deficient in UMPS. Subsequent biochemical tests showed that these cows had only 50% of the normal activity of this enzyme (reviewed by Shanks and Robinson, 1990). Inheritance and molecular studies finally brought the whole story to light.

Inheritance

DUMPS is an autosomal recessive embryonic lethal.

Molecular Genetics

By cloning and sequencing a very likely candidate gene (based on knowledge that the bovine disorder is due to deficiency of uridine monophosphate sythetase), Schwenger et al. (1993) identified a C>T transition in the bovine UMPS gene as a causal mutation.

Genetic Test

Knowledge that the causal mutation removes a recognition sequence for the restriction enzyme AvaI immediately gave rise to a DNA test.

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