1.0 2018-05-07 22:20:25 UTC 2020-05-20 21:10:15 UTC BMDBP00014 BMDBP03108 Enzyme CYP51A1 Secondary metabolites biosynthesis, transport and catabolism A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Steroid Biosynthesis SMP0087233 Zymosterol biosynthesis Zymosterol from lanosterol: step 1/6 BMDB0000142 Formic acid BMDB0000217 NADP BMDB0000221 NADPH BMDB0000692 Iron BMDB0001242 Obtusifoliol BMDB0003178 Heme BMDB0001251 Lanosterin BMDB0001377 Oxygen BMDB0001023 4,4-Dimethylcholesta-8,14,24-trienol BMDB0002111 Water BMDB0001419 24-Hydroxycholesterol BMDB0011644 (24R)-Cholest-5-ene-3-beta,7-alpha,24-triol 4 >1509 bp ATGCTGGACTTACTGCAGGCGGGCGGGTCAGTGCTGGGGCAGGCGATGGAGCAAGTAACG GGCGGCAACCTCGCATCCATGCTGCTCATCGCCTGCGCCTTCACGCTTAGTCTGGTCTAC CTGTTCCGCCTCGCGGTTGGCCACCTGGCCCCACCACTGCCGACTGGGGCGAAAAGTCCA CCATACATTGTCTCTCCAATTCCATTCCTTGGACATGCTATAGCATTTGGGAAAAGTCCA ATTGAATTCCTAGAAGATGCATATGAGAAGTATGGACCTGTATTTAGTTTTACCATGGTG GGAAAGACGTTTACCTACCTGCTGGGGAGTGAGGCTGCTGCACTGCTTTTTAATAGTAAA AATGAAGACTTGAATGCCGAGGAAGTCTACAGTCGTCTGACGACACCTGTGTTTGGGAAG GGAGTTGCCTATGATGTGCCTAATACAGTTTTCTTGGAGCAGAAGAAAATGTTGAAAAGT GGTCTTAACATAGCCCACTTTAGACAGCACGTGTCTATAATTGAAAAAGAAACAAAAGAA TACTTTAAAAGTTGGGGAGAAAGTGGAGAAAAAAATTTGTTTGAAGCTCTTTCTGAGCTC ATCATTTTAACAGCCAGCCATTGTTTACATGGAAAGGAAATCAGAAGTCAACTCAACGAG AAGGTGGCACAACTGTATGCGGATTTGGATGGAGGTTTTAGCCATGCAGCCTGGCTTTTG CCAGGCTGGCTCCCTCTGCCTAGTTTCAGACGCAGGGACAGAGCTCATCGGGAGATCAAG AATATTTTCTACAAGGCAATCCAGAAACGCAGAGAGTCAGGAGAAAAAATTGATGACATT CTCCAAACTTTACTAGAGTCTACTTACAAGGATGGGCGTCCTCTGACGGATGATGAAGTA GCAGGCATGCTTATTGGACTGCTCTTGGCAGGGCAGCATACTTCTTCAACTACCAGTGCC TGGATGGGCTTCTTTTTGGCCAGAGACAAAACACTTCAAGAAAAATGTTTCTTAGAACAG AAAACAGTCTGTGGTGAGAATCTGCCTCCTCTGACTTATGACCAGCTCAAGGATCTCAAT TTACTTGATCGCTGTATAAAAGAAACATTAAGACTTCGACCTCCTATAATGACCATGATG AGACTGGCCAAAACTCCTCTGACTGTGGCAGGGTACACCATTCCTCCAGGACACCAGGTG TGTGTGTCTCCTACTGTCAATCAGAGACTTAAAGACTCATGGGTAGAGCGTCTGGACTTC AATCCTGACCGCTACTTAGAGGACAGCCCAGCATCAGGAGAGAAGTTTGCTTACGTGCCA TTTGGAGCTGGGCGTCATCGTTGCATTGGGGAGAATTTTGCCTATGTTCAAATCAAGACA ATTTGGTCCACTATGCTTCGTTTATATGAATTTGATCTCATTGATGGGTATTTTCCCACT GTGAATTATACAACTATGATTCACACCCCTGAAAAACCCATCATCAGATACAAACGAAGA TCAAAATGA 502 56596.0 8.25 22-42 None >Lanosterol 14-alpha demethylase MLDLLQAGGSVLGQAMEQVTGGNLASMLLIACAFTLSLVYLFRLAVGHLAPPLPTGAKSP PYIVSPIPFLGHAIAFGKSPIEFLEDAYEKYGPVFSFTMVGKTFTYLLGSEAAALLFNSK NEDLNAEEVYSRLTTPVFGKGVAYDVPNTVFLEQKKMLKSGLNIAHFRQHVSIIEKETKE YFKSWGESGEKNLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLL PGWLPLPSFRRRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYKDGRPLTDDEV AGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQLKDLN LLDRCIKETLRLRPPIMTMMRLAKTPLTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDF NPDRYLEDSPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPT VNYTTMIHTPEKPIIRYKRRSK AAY82452.1 Q4PJW3 CP51A_BOVIN DQ078267 CYP51A1