| Identification |
|---|
| BMDB Protein ID
| BMDBP00411 |
| Secondary Accession Numbers
| None |
| Name
| Protein arginine N-methyltransferase 5 |
| Synonyms
|
Not Available
|
| Gene Name
| PRMT5 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in histone-arginine N-methyltransferase activity |
| Specific Function
| Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties (By similarity). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity). Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (By similarity). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter (By similarity). Methylates GM130/GOLGA2, regulating Golgi ribbon formation. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner. Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. Along with LYAR, binds the promoter of gamma-globin HBG1/HBG2 and represses its expression. Symmetrically methylates NCL (By similarity). Methylates TP53; methylation might possibly affect TP53 target gene specificity (By similarity). Involved in spliceosome maturation and mRNA splicing in prophase I spermatocytes through the catalysis of the symmetrical arginine dimethylation of SNRPB (small nuclear ribonucleoprotein-associated protein B) and the interaction with tudor domain-containing protein TDRD6 (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 10 |
| Locus
| Not Available |
| SNPs
| PRMT5 |
| Gene Sequence
|
>1914 bp
ATGGCGGCGATGGCGGTCGGTGGTGCCGGTGGAAGCCGCGTGTCCAGCGGGAGGGACCTG
AATTGCGTCCCCGAAATAGCTGACACACTGGGGGCTGTGGCCAAGCAGGGGTTTGATTTC
CTCTGCATGCCTGTGTTCCACCCGCGTTTCAAGAGGGAATTCACTCAGGAACCTGCTAAG
AGTCGGCCGGGCCCCCAGACACGATCAGACCTACTGCTGTCAGGAAGGGACTGGAATACA
CTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAAAGTGGAGAAGATACGC
AGGAACTCTGAGGCGGCTATGTTACAGGAGCTGAATTTTGGGGCATATTTGGGTCTTCCA
GCTTTCCTGCTGCCCCTAAATCAGGAAGATAACACAAACTTGGCGAGAGTTTTGACCAAT
CACATCCACACTGGCCACCACTCCTCCATGTTCTGGATGCGGGTGCCATTGGTGGCACCA
GAGGACCTGAGAGATGATATAATTGAGAACGCACCAACTTCACACACAGAGGAGTACAGT
GGAGAGGAGAAGACATGGATGTGGTGGCACAACTTCCGGACCTTATGTGATTACAGCAAG
AGGATTGCAGTGGCTCTTGAAATTGGTGCTGACCTCCCATCTAATCATGTCATTGATCGT
TGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTAGCATTTTCCTGACCAATAAG
AAGGGATTTCCTGTTCTTTCTAAGATGCACCAGAGGTTGATCTTCCGACTTCTCAAGTTG
GAGGTACAGTTCATCATCACAGGCACCAACCACCACTCAGAGAAGGAGTTCTGCTCCTAC
CTCCAATACTTGGAATACCTGAGCCAGAACCGACCTCCACCCAATGCCTACGAACTCTTT
GCCAAGGGCTATGAAGATTACCTGCAGTCCCCACTCCAGCCACTGATGGATAACCTGGAA
TCTCAGACATACGAAGTGTTCGAAAAGGACCCCATCAAATACTCTCAGTACCAGCAGGCC
ATCTATAAATGTCTGTTAGATCGAGTGCCAGAGGAAGAGAAGGACACCAATATCCAAGTG
CTGATGGTGCTGGGAGCAGGCCGGGGGCCCCTGGTGAATGCTTCCCTGCGGGCCGCCAAG
CAGGCTGACCGGCGGATAAAATTGTACGCTGTGGAGAAGAACCCAAATGCTGTGGTGACG
TTGGAGAACTGGCAGTTTGAAGAATGGGGAAGCCAGGTGACAGTAGTCTCATCGGACATG
CGGGAGTGGGTGGCTCCAGAGAAAGCAGATATCATTGTCAGTGAGCTTCTGGGGTCCTTT
GCTGACAATGAACTGTCACCTGAGAGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGAT
GGCGTGAGCATTCCTGGGGAGTACACCTCCTTTCTAGCTCCCATCTCCTCCTCCAAGCTA
TACAATGAGGTCCGAGCCTGTCGGGAAAAGGACCGTGACCCTGAGGCCCAGTTTGAGATG
CCTTATGTGGTACGACTGCACAATTTCCACCAGCTGTCTGCACCCCAGCCCTGTTTTACC
TTCAGCCATCCTAACAGAGATCCTATGATTGACAACAATCGCTACTGTACCTTGGAGTTT
CCTGTGGAGGTGAACACAGTGCTGCATGGTTTTGCAGGCTACTTTGAGACTGTGCTTTAT
CAGGACATCACTCTGAGTATCCGTCCAGAGACTCACTCTCCTGGGATGTTTTCATGGTTT
CCCATCCTCTTCCCCATTAAGCAGCCCATTACGGTGCGTGAAGGCCAGACCATCTGTGTG
CGTTTCTGGCGATGCAGCAACTCTAAGAAGGTGTGGTATGAGTGGGCTGTGACGGCACCG
GTCTGCTCTGCTATTCACAACCCCACAGGCCGCTCTTACACCATTGGCCTCTAG
|
| Protein Properties |
|---|
| Number of Residues
| 637 |
| Molecular Weight
| 72629.0 |
| Theoretical pI
| 6.24 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Protein arginine N-methyltransferase 5
MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFTQEPAK
SRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLP
AFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPTSHTEEYS
GEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNK
KGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELF
AKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPEEEKDTNIQV
LMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDM
REWVAPEKADIIVSELLGSFADNELSPESLDGAQHFLKDDGVSIPGEYTSFLAPISSSKL
YNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEF
PVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICV
RFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL
|
| External Links |
|---|
| GenBank ID Protein
| AAI34450.1 |
| UniProtKB/Swiss-Prot ID
| A7YW45 |
| UniProtKB/Swiss-Prot Entry Name
| ANM5_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC134449 |
| GeneCard ID
| PRMT5 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
