| Identification |
|---|
| BMDB Protein ID
| BMDBP00665 |
| Secondary Accession Numbers
| None |
| Name
| Peptidyl-glycine alpha-amidating monooxygenase |
| Synonyms
|
Not Available
|
| Gene Name
| PAM |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in copper ion binding |
| Specific Function
| Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (PubMed:2059626). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:2059626). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:2059626). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 7 |
| Locus
| Not Available |
| SNPs
| PAM |
| Gene Sequence
|
>2919 bp
ATGGCTGGCTTTCGGAGCCTGCTAGTTCTTCTCCTCGTGTTTCCCAGTGGCTGTGTGGGC
TTCCGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGATCATTTTCCAAT
GAATGTCTTGGTACCACCAGACCAGTAATTCCTATCGATTCATCAGATTTTGCGTTGGAT
ATTCGCATGCCTGGGGTCACACCCAAACAGTCTGATACGTACTTCTGCATGTCAGTGCGT
TTGCCAATGGATGAGGAAGCCTTCGTGATTGACTTCAAACCCCGTGCCAGCATGGATACT
GTCCATCATATGTTACTGTTTGGATGCAATATGCCAGCGTCCACTGGAAATTACTGGTTT
TGTGATGAAGGCACCTGTACAGATAAAGCCAATATTCTCTATGCCTGGGCAAGAAATGCT
CCCCCAACGAGACTCCCCAAAGGTGTTGGATTCAGAGTTGGAGGAGAGACTGGAAGCAAA
TACTTTGTACTCCAAGTACACTATGGGGATATTAGTGCTTTTAGAGATAATCACAAGGAC
TGCTCTGGTGTTTCCTTACACCTCACACGCCTGCCACAGCCTTTAATTGCTGGCATGTAC
CTTATGATGTCTGTTGACACTGTTATACCACCAGGAGGGAAAGTGGTGAATTCTGATATT
TCATGCCATTATAAAAAGTATCCAATGCATGTCTTTGCCTACAGAGTTCACACTCACCAT
TTAGGCAAGGTAGTCAGTGGATACAGAGTAAGAAATGGACAGTGGACCCTGATTGGACGC
CAAAGCCCCCAACTGCCACAGGCCTTCTACCCAGTAGAGCACCCAGTGGATGTGAGTTTT
GGTGACATCCTGGCAGCAAGATGTGTGTTCACTGGGGAAGGGAGGACAGAAGTCACACAC
ATTGGTGGCACGTCTAGTGATGAAATGTGCAACTTATACATTATGTATTACATGGAAGCC
AAGCATGCAGTTTCTTTCATGACCTGTACACAGAATGTAGCTCCAGATATATTCAGAACC
ATACCACCAGAGGCCAATATTCCAATTCCTGTGAAATCCGATATGGTTATGATGCATGGA
CATCACAAAGAAACAGAGAACAAAGACAAGACTTCTTTACTACAGCAGCCCAAACGAGAA
GAAGAAGGAGTGTTAGAACAGGGTGATTTCTATTCACTACTTTCCAAGCTGCTAGGAGAA
AGGGAAGATGTTGTTCATGTGCATAAATATAATCCTACAGAAAAGGCAGAATCAGAGTCT
GACCTGGTAGCTGAGATTGCAAACGTAGTCCAAAAGAAGGATCTTGGTCGGTCCGATACC
AGAGAGAGTGCAGAACAGGAGAGGGGCAATGCTATTCTTGTCAGAGACAGAATTCACAAA
TTCCACAGACTAGTGTCTACCTTGAGGCCTGCAGAGAGCAGAGTTCTGTCGTTACAGCAG
CCCCTACCTGGTGAAGGCACCTGGGAACCAGAACACACAGGAGATTTCCATGTAGAAGAG
GCACTGGATTGGCCTGGAGTATACTTGTTACCAGGCCAGGTTTCTGGGGTGGCTCTGGAC
CCTCAGAATAATCTCGTGATTTTCCACCGAGGTGACCACGTCTGGGATGGAAACTCTTTT
GATAGCAAGTTTGTTTACCAGCAAAGAGGTCTTGGACCAATTGAAGAAGACACTATTCTT
GTCATAGATCCAAATAATGCTGCAGTACTCCAGTCCAGTGGAAAAAATCTGTTTTACTTG
CCACATGGCTTGAGTATAGATAAAGATGGAAATTATTGGGTCACAGACGTGGCGCTTCAT
CAGGTGTTCAAACTAGATCCAAAGAGTAAAGAAGGCCCTCTGCTAACCCTGGGAAGGAGC
ATGCAACCAGGCAGTGACCAGAATCACTTCTGTCAGCCCACCGATGTGGCTGTGGATCCA
GACACCGGAACCATCTATGTGTCAGATGGCTACTGCAACAGTCGCCTTGTGCAGTTTTCA
CCAAGTGGAAAATTCATCACACAGTGGGGAGAAGCGTCTCTAGAGAGCAGTCCTAAACCA
GGCCAGTTCAGAGTTCCTCACAGCTTGGCCCTGGTGCCTCCCCTGGGCCAGCTGTGTGTG
GCAGACCGGGAAAACGGTCGGATCCAGTGTTTCAAAACCGACACCAAAGAATTTGTGCGC
GAGATTAAGCACCCATCGTTTGGAAGAAATGTGTTTGCAATTTCATACATACCAGGTTTG
CTCTTTGCGGTGAATGGAAAGCCTTACTTTGAGGACCAAGAACCCGTGCAAGGATTTGTG
ATGAACTTTTCCAGCGGGGAAATTATCGATGTCTTCAAGCCAGTGCGCAAGCACTTCGAC
ATGCCCCACGACATTGCTGCGTCCGAGGACGGGACCGTGTATGTCGGAGACGCTCACACC
AACACCGTGTGGAAGTTCACCTCGACCGAAAAAATGGAACATCGATCAGTTAAGAAGGCT
GGCATTGAGGTTCAGGAAATCAAAGAATCCGAGGCAGTTGTTGAAACCAAAATGGAGAAC
AAGCCCGCCTCCTCAGAATTGCAGAAGATACAAGAGAAACAGAAGCTGGTCAAAGAGCCG
GGCTCCGGAGTGCCGGCTGTTCTCATTACAACCCTTCTGGTTATTCCTGTGGTTGTCCTG
CTGGCCATTGCCTTATTTATTCGGTGGAAAAAATCAAGGGCCTTTGGAGATTCTGAACGT
AAACTTGAGGCCAGTTCGGGAAGAGTTCTGGGAAGACTTAGAGGAAAGGGAGGCGGAGGC
CTAAACCTGGGCAACTTCTTCGCCAGCCGGAAGGGCTACAGCCGGAAGGGGTTCGACCGC
CTCAGCACGGAAGGAAGCGACCAGGAGAAAGATGAGGACGCAAGCGAGTCCGAAGAAGAG
TACTCGGCCCCGCCGCCCGCCCCTGCCCCTTCCTCCTGA
|
| Protein Properties |
|---|
| Number of Residues
| 972 |
| Molecular Weight
| 108177.0 |
| Theoretical pI
| 6.59 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Peptidyl-glycine alpha-amidating monooxygenase
MAGFRSLLVLLLVFPSGCVGFRSPLSVFKRFKETTRSFSNECLGTTRPVIPIDSSDFALD
IRMPGVTPKQSDTYFCMSVRLPMDEEAFVIDFKPRASMDTVHHMLLFGCNMPASTGNYWF
CDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKD
CSGVSLHLTRLPQPLIAGMYLMMSVDTVIPPGGKVVNSDISCHYKKYPMHVFAYRVHTHH
LGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVSFGDILAARCVFTGEGRTEVTH
IGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDIFRTIPPEANIPIPVKSDMVMMHG
HHKETENKDKTSLLQQPKREEEGVLEQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESES
DLVAEIANVVQKKDLGRSDTRESAEQERGNAILVRDRIHKFHRLVSTLRPAESRVLSLQQ
PLPGEGTWEPEHTGDFHVEEALDWPGVYLLPGQVSGVALDPQNNLVIFHRGDHVWDGNSF
DSKFVYQQRGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALH
QVFKLDPKSKEGPLLTLGRSMQPGSDQNHFCQPTDVAVDPDTGTIYVSDGYCNSRLVQFS
PSGKFITQWGEASLESSPKPGQFRVPHSLALVPPLGQLCVADRENGRIQCFKTDTKEFVR
EIKHPSFGRNVFAISYIPGLLFAVNGKPYFEDQEPVQGFVMNFSSGEIIDVFKPVRKHFD
MPHDIAASEDGTVYVGDAHTNTVWKFTSTEKMEHRSVKKAGIEVQEIKESEAVVETKMEN
KPASSELQKIQEKQKLVKEPGSGVPAVLITTLLVIPVVVLLAIALFIRWKKSRAFGDSER
KLEASSGRVLGRLRGKGGGGLNLGNFFASRKGYSRKGFDRLSTEGSDQEKDEDASESEEE
YSAPPPAPAPSS
|
| External Links |
|---|
| GenBank ID Protein
| AAA30683.1 |
| UniProtKB/Swiss-Prot ID
| P10731 |
| UniProtKB/Swiss-Prot Entry Name
| AMD_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| M18683 |
| GeneCard ID
| PAM |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
