| Identification |
|---|
| BMDB Protein ID
| BMDBP00825 |
| Secondary Accession Numbers
| None |
| Name
| Aprataxin and PNK-like factor |
| Synonyms
|
Not Available
|
| Gene Name
| APLF |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in 3'-5' exonuclease activity |
| Specific Function
| Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 11 |
| Locus
| Not Available |
| SNPs
| APLF |
| Gene Sequence
|
>1458 bp
ATGTCCGGGGGCTTCGAGCTGCAGCCGCAGGACGGCGGCCCCCGGGTGGCCTTGGCGCCC
GGGGAGACGGTCATCGGCCGCGGGCCGCTTCTGGGACTACACAGAAATCCATGTTATTAT
CAGTCTTCTGAGAAGAGCCAGCTCTTACCATTGAAGACAAATATATGGTGCTGGTTGAAT
CCTGGAGATCACTTTTCTTTATTAGTTGACAAATACATTTTCTGTGTCTTGTCTACACAT
TCTGAAATGGAAATGGAGTGTACTTTAAGAAATAGTCAAATGCTTGATGAAGATGATATA
TTGAATGAAATACCAAAATCTTCCTCAGCTGATTTGCCTGATAAAACACCCAGTGCCCCA
CGGCGGGAAAGAAGTACAGAAACAGCAAAGCCCCAGGCCGCTGCAAATAACATGTCTTTC
ATAGGTGAAAGTAGAGACTTGAGTAAGCAACAGCCAAATCCTTCCGAGAGGAAAAGAATC
CTCCCAGCATGGATGTTAACAGAAAATTCAAGTGATCAGAACCTTTCAGTCATCAGTGGA
GGTAATAATGTAACCTGGGAAAGTGAAAAAGAAAGAGTCTGCAAAGATAAAACCCAAGTA
AACATAACACAGCCAGGAAAAAAGCGATTAATTTCATCAGGAAGTTCAGAAAGTACATCA
GCAAAGCAAGACACAGGAAAAAAGTGCAAAAATGATGATCAGGAAGAGTCTATCATTTCA
TCCAAGGAAATGCCACAGTCATTTTCTGCAGCCATGTTACATAACACAGAGATAGATAAC
ACTAAGACTAATCCACAGAGAAGCAAAGTCCCAGTAGAGGCACTTGGTAAGGTTTCTGAA
CATAAAATCATCACTAAGGGAAGTTCAAATGAAGACAGCACGGCGAGAAGTTGTTCTGAG
AGTTATTCCAGTACCCAGAGCAAGTCTTTCTGTGACAAGCCTCAAAAATCTCATCCTGAG
CCCAGCTCTAATCCGCCCAGTCCTGAATGTGTGCAGGCGAAGGCAACCGATTCAGTTCCA
AATGGTTCTGAAGAAAACAAGGTACAGAGAACATCCTGCATGTATGGGGCAAACTGCTAC
AGGAAAAATCCTGTCCATTTTCAACACTTCAGTCACCCTGGTGATAGTGATTATGGAGGT
GTAAACATCACGTGTCAAGATGAAGCTGATGACCGACCTGAATGTCCCTATGGAGCATCT
TGTTATAGGAAGAATCCCCAGCACAAGATAGAATATAGACATAGTACATTTCCAGTGAGA
AGCATTTCAGATGAAGATGACAATGTTGGGCAACCCAATGAATACAACCTGAATGACAGC
TTTATAGATGATGAGGAAGAAGAATACGAGCCAACAGACGAAGATTCCGACTGGGAACCA
GAAAAGGAAGACCTAGAGAAGGAAGATATGGAAGGGCTTTTGAAAGAAGCAAAAAAATTT
ATGAAAAGAAAAAAGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 485 |
| Molecular Weight
| 54326.0 |
| Theoretical pI
| 4.87 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Aprataxin and PNK-like factor
MSGGFELQPQDGGPRVALAPGETVIGRGPLLGLHRNPCYYQSSEKSQLLPLKTNIWCWLN
PGDHFSLLVDKYIFCVLSTHSEMEMECTLRNSQMLDEDDILNEIPKSSSADLPDKTPSAP
RRERSTETAKPQAAANNMSFIGESRDLSKQQPNPSERKRILPAWMLTENSSDQNLSVISG
GNNVTWESEKERVCKDKTQVNITQPGKKRLISSGSSESTSAKQDTGKKCKNDDQEESIIS
SKEMPQSFSAAMLHNTEIDNTKTNPQRSKVPVEALGKVSEHKIITKGSSNEDSTARSCSE
SYSSTQSKSFCDKPQKSHPEPSSNPPSPECVQAKATDSVPNGSEENKVQRTSCMYGANCY
RKNPVHFQHFSHPGDSDYGGVNITCQDEADDRPECPYGASCYRKNPQHKIEYRHSTFPVR
SISDEDDNVGQPNEYNLNDSFIDDEEEEYEPTDEDSDWEPEKEDLEKEDMEGLLKEAKKF
MKRKK
|
| External Links |
|---|
| GenBank ID Protein
| AAI26692.1 |
| UniProtKB/Swiss-Prot ID
| A0JNH9 |
| UniProtKB/Swiss-Prot Entry Name
| APLF_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC126691 |
| GeneCard ID
| APLF |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
