| Identification |
|---|
| BMDB Protein ID
| BMDBP01054 |
| Secondary Accession Numbers
| None |
| Name
| Cleavage and polyadenylation specificity factor subunit 3 |
| Synonyms
|
Not Available
|
| Gene Name
| CPSF3 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Translation, ribosomal structure and biogenesis |
| Specific Function
| Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 11 |
| Locus
| Not Available |
| SNPs
| CPSF3 |
| Gene Sequence
|
>2055 bp
ATGTCCGCGATTCCTGCAGAGGAGAGTGACCAGCTGCTGATCCGACCCCTTGGAGCTGGG
CAGGAAGTGGGAAGATCATGTATTATTCTGGAGTTCAAGGGGAGGAAGATAATGCTGGAC
TGTGGGATCCATCCTGGCCTAGAAGGAATGGATGCTCTTCCTTATATTGATTTGATTGAC
CCAGCTGAGATTGATCTCCTACTAATTAGTCATTTCCATTTGGATCACTGTGGAGCTTTG
CCCTGGTTTCTACAGAAGACAAGTTTCAAAGGAAGAACATTTATGACTCATGCTACAAAA
GCTATTTATAGATGGCTTCTTTCAGATTATGTCAAAGTTAGTAACATATCAGCAGACGAC
ATGCTGTATACTGAGACAGATTTGGAAGAAAGCATGGACAAAATTGAAACCATCAATTTT
CATGAAGTGAAGGAAGTTGCAGGAATCAAGTTTTGGTGTTATCACGCCGGCCATGTCCTG
GGAGCTGCCATGTTCATGATCGAGATCGCAGGCGTGAAGCTTTTATACACAGGTGATTTC
TCAAGACAAGAAGACAGACACTTAATGGCAGCTGAAATTCCTAACATTAAACCTGATATT
CTTATCATTGAATCTACTTATGGGACCCATATCCACGAGAAGCGAGAGGAGCGAGAGGCA
AGGTTCTGTAATACCGTCCATGATATTGTGAACAGAGGGGGCCGAGGCCTCATTCCYGTC
TTTGCYCTCGGACGGGCCCAGGAACTCCTCTTGATTTTAGATGAGTACTGGCAGAAYCAC
CCAGAACTCCATGATATTCCAATATACTATGCATCATCTTTGGCCAAGAAGTGTATGGCA
GTGTACCAGACATATGTGAAYGCCATGAATGACAAAATCCGCAAACAGATCAACATCAAT
AATCCATTTGTTTTCAAACACATTAGTAATCTGAAGAGCATGGATCATTTTGATGACATT
GGTCCCAGCGTGGTCATGGCCTCCCCAGGCATGATGCAAAGTGGTTTATCCAGAGAGCTC
TTTGAAAGCTGGTGTACTGATAAGAGGAATGGCGTCATCATAGCCGGCTACTGTGTAGAA
GGCACGCTTGCCAAGCATATCATGTCTGAACCGGAAGAAATCACTACCATGTCCGGGCAG
AAGTTACCTCTGAAAATGTCTGTTGATTACATCTCTTTCTCTGCGCACACGGATTACCAG
CAAACCAGTGAATTTATACGTGCTTTGAAGCCGCCTCAYGTGATYTTAGTCCATGGAGAA
CAGAATGAAATGGCCAGATTGAAAGCAGCCCTGATTCGAGAATATGAAGATAATGATGAR
GTTCACATAGAGGTTCATAATCCTCGGAATACAGAGGCAGTGACCTTGAACTTCAGGGGA
GAAAAACTAGCCAAGGTCATGGGATTTTTAGCAGACAAAAAACCAGAACAGGGCCAGCGG
GTCTCAGGAATACTTGTTAAAAGAAACTTTAATTATCACATACTTTCTCCTTGTGACCTC
TCCAATTATACTGACTTGGCCATGAGCACTGTAAAACAGACCCAAGCCATTCCATATACT
GGTCCTTTTAATTTGCTCTATTACCAGCTACAGAAATTGACAGGTGATGTGGAAGAATTA
GAAATTCAAGAAAAGCCTGCTTTGAAAGTGTTTAAAAATATTACTGTAATACAGGAACCA
GGAATGGTGGTATTAGAATGGTTGGCAAATCCTTCCAATGATATGTATGCAGATACAGTC
ACAACCGTGATACTAGAAGTTCAGTCAAACCCAAAAATAAGGAAAGGTGCAGTACAGAAG
GTTTCTAAAAAATTAGAAATGCACGTTTACAGCAAGAGGCTGGAGATCATGCTCCAGGAC
ATATTTGGAGAAGACTGTGTTAGTGTAAAAGATGGTTCTATTCTTAGTGTCACAGTGGAT
GGAAAAACTGCTAACATAAATTTGGAGACACGGACTGTAGAATGTGAAGAAGGCAGTGAA
GATGACGAGTCCCTCCGAGAAATGGTGGAACTGGCGGCCCAGAGACTGTACGAGGCCCTC
ACACCCGTCCACTGA
|
| Protein Properties |
|---|
| Number of Residues
| 684 |
| Molecular Weight
| 77488.0 |
| Theoretical pI
| 5.41 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Cleavage and polyadenylation specificity factor subunit 3
MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLID
PAEIDLLLISHFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADD
MLYTETDLEESMDKIETINFHEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDF
SRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEKREEREARFCNTVHDIVNRGGRGLIPV
FALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMAVYQTYVNAMNDKIRKQININ
NPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRNGVIIAGYCVE
GTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGE
QNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQR
VSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLYYQLQKLTGDVEEL
EIQEKPALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQK
VSKKLEMHVYSKRLEIMLQDIFGEDCVSVKDGSILSVTVDGKTANINLETRTVECEEGSE
DDESLREMVELAAQRLYEALTPVH
|
| External Links |
|---|
| GenBank ID Protein
| CAA65151.1 |
| UniProtKB/Swiss-Prot ID
| P79101 |
| UniProtKB/Swiss-Prot Entry Name
| CPSF3_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| X95906 |
| GeneCard ID
| CPSF3 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
