| Identification |
|---|
| BMDB Protein ID
| BMDBP01071 |
| Secondary Accession Numbers
| None |
| Name
| E3 ubiquitin-protein ligase BRE1A |
| Synonyms
|
Not Available
|
| Gene Name
| RNF20 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Replication, recombination and repair |
| Specific Function
| Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of PA2G4 leading to its proteasome-mediated degradation. |
| Pathways
|
- Protein modification
- Protein ubiquitination
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 8 |
| Locus
| Not Available |
| SNPs
| RNF20 |
| Gene Sequence
|
>2928 bp
ATGTCAGGAATTGGAAGTAAAAGAGCAGCTGGAGAGCCTGGCACATCTGTGCCTCCAGAG
AAGAAGACAGCTGTTGAAGATTCAGGGACCACAGTGGAAACAATTAAACTTGGGGGTGTC
TCTTCAACGGAGGAGTTAGACATTCGAACACTGCAAACCAAAAATCGCAAACTGGCAGAA
ATGCTGGATCAACGGCAAGCCATCGAAGATGAACTCCGTGAACACATTGAAAAACTGGAA
CGAAGACAGGCCACTGATGATGCCTCACTGTTGATTGTCAACCGATACTGGAGTCAGTTT
GATGAAAACATCCGTATCATCCTTAAGCGTTATGATCTGGAGCAAGGTTTGGGAGACCTA
CTCACAGAGAGAAAAGCCCTTGTTGTGCCCGAGCCAGAACCAGACTCTGATAGCAATCAA
GAACGGAAAGATGACCGAGAGAGAGGGGAAGGGCAAGAGCCAGCTTTCTCTTTCCTTGCT
ACTTTGGCCAGCAGTTCCAGTGAAGAGATGGAATCTCAGCTGCAGGAGCGCGTGGAGTCC
TCCCGCCGAGCGGTGTCCCAGATTGTGACCGTCTATGATAAACTGCAAGAAAAGGTGGAG
CTCTTATCACGGAAGCTGAACAGTGGAGACAGTCTGATGGTAGAGGAAGCAGTGCAGGAG
CTGAATTCCTTCCTTGCACAAGAGAACACAAGGCTGCAAGAACTGACAGACCTCCTTCAG
GAGAAGCATTGCACCATGTCTCAGGAGTTTTCCAAGTTGCAGAGTAAAGTGGAGACAGCT
GAGTCACGAGTGTCTGTCCTGGAGTCCATGATCGATGACCTGCAGTGGGATATTGACAAA
ATTCGAAAGCGGGAACAGCGACTTAACCGACACTTAGCAGAAGTCTTAGAACGGGTAAAT
TCAAAAGGTTATAAAGTGTACGGTGCAGGGAGCAGCCTCTATGGTGGTACAATCACCATC
AACGCCCGGAAGTTTGAGGAAATGAATGCGGAGCTTGAAGAGAACAAAGAGTTGGCTCAG
AACCGTCACTGTGAGCTGGAGAAACTTCGGCAAGACTTCGAGGAGGTCACTTCACAAAAT
GAAAAACTGAAGGTGGAATTACGGAGCGCAGTGGAGGAAGTGGTCAAGGAGACTCCAGAA
TATCGCTGCATGCAGTCACAGTTCTCCGTGCTGTACAACGAGAGTCTGCAGTTGAAAGCA
CACTTGGATGAGGCTCGGACCCTGCTTCATGGCACTAGGGGAACCCACCAACGTCAGGTG
GAGCTCATTGAGCGAGATGAGGTTAGTCTTCATAAGAAGCTGAGGACGGAAGTAATCCAG
CTGGAAGATACACTGGCCCAGGTCCGCAAGGAGTATGAGATGCTGAGGATAGAATTCGAG
CAGACCCTTGCTGCCAATGAACAAGCAGGCCCCATAAACCGGGAGATGCGCCACCTCATC
AGTAGCCTCCAGAATCACAACCACCAGCTGAAAGGGGAGGTCCTAAGGTATAAGCGGAAA
CTGAGAGAAGCCCAGTCTGACCTGAACAAGACTCGGCTGCGCAGTGGCAGTGCCCTCCTG
CAGTCTCAGTCCAGTACTGAGGACCCTAAGGACGAGCCTGCAGAGCTAAAACAGGATTCT
GAGGACTTAGCCACCCAGTCTGCTGCATCGAAGGCATCTCAGGAGGAGGTCAACGAAATT
AAGTCCAAACGCGATGAGGAAGAGCGAGAACGAGAAAGGAGGGAGAAAGAGAGGGAGCGA
GAAAGAGAGCGGGAGAAGGAGAAGGAGCGAGAACGAGAGAAGCAGAAACTAAAAGAGTCA
GAAAAAGAGAGAGAGTCTGCTAAGGATAAAGAGAAAGGGAAACATGATGATGGAAGGAAA
AAGGAAGCGGAAATTATCAAACAATTGAAGATTGAACTCAAGAAAGCACAGGAGAGCCAA
AAGGAGATGAAACTGTTGCTGGATATGTACCGCTCTGCCCCAAAGGAGCAGAGAGACAAG
GTTCAGCTGATGGCAGCAGAGAAGAAGTCTAAGGCAGAGTTGGAAGATTTAAGACAAAGA
CTCAAGGATCTGGAGGATAAGGAGAAAAAAGAGAATAAGAAAATGGCTGATGAAGATGCC
TTAAGAAAGATCCGGGCAGTAGAGGAGCAGATAGAATACCTCCAGAAGAAGCTGGCCATG
GCCAAGCAGGAAGAAGAAGCTCTCCTCTCTGAGATGGACGTCACAGGCCAGGCCTTTGAA
GATATGCAGGAGCAAAATATCCGTTTGATGCAGCAGCTGAGGGAGAAGGATGATGCAAAC
TTCAAGCTCATGTCAGAGCGTATCAAATCCAATCAGATCCATAAGCTGCTGAAAGAAGAG
AAGGAGGAGCTGGCAGACCAAGTTTTGACTCTGAAGACTCAGGTTGATGCCCAGTTACAG
GTAGTGAGGAAACTGGAAGAGAAGGAGCACCTGTTGCAGAGCAACATCGGCACAGGGGAG
AAGGAGCTGGGTCTTAGGACCCAGGCCTTGGAGATGAATAAACGCAAGGCAATGGAGGCA
GCCCAGCTTGCAGATGACCTCAAAGCACAGTTGGAGATGGCTCAGAAGAAGCTACATGAT
TTTCAGGATGAGATTGTAGAGAACAGTGTCACCAAAGAAAAAGATATGTTCAATTTCAAA
CGGGCCCAGGAGGACATCTCTAGACTTCGAAGGAAGCTGGAGACCACGAAGAAACCAGAC
AATGTACCCAAATGTGATGAGATTCTGATGGAGGAGATTAAGGACTACAAGGCACGCCTG
ACCTGTCCGTGTTGCAACATGCGTAAAAAGGATGCTGTGCTTACCAAGTGTTTTCATGTT
TTCTGCTTTGAGTGTGTTAAGACCCGCTATGATACCCGCCAGCGCAAATGTCCCAAGTGT
AATGCTGCCTTTGGTGCCAACGATTTCCATCGCATCTACATAGGTTGA
|
| Protein Properties |
|---|
| Number of Residues
| 975 |
| Molecular Weight
| 113658.0 |
| Theoretical pI
| 5.64 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>E3 ubiquitin-protein ligase BRE1A
MSGIGSKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAE
MLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDL
LTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVES
SRRAVSQIVTVYDKLQEKVELLSRKLNSGDSLMVEEAVQELNSFLAQENTRLQELTDLLQ
EKHCTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVN
SKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTSQN
EKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQV
ELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLI
SSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDS
EDLATQSAASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKES
EKERESAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDK
VQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAM
AKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEE
KEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEA
AQLADDLKAQLEMAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPD
NVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKC
NAAFGANDFHRIYIG
|
| External Links |
|---|
| GenBank ID Protein
| AAI33335.1 |
| UniProtKB/Swiss-Prot ID
| A2VDP1 |
| UniProtKB/Swiss-Prot Entry Name
| BRE1A_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC133334 |
| GeneCard ID
| RNF20 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
