| Identification |
|---|
| BMDB Protein ID
| BMDBP01567 |
| Secondary Accession Numbers
| None |
| Name
| Microprocessor complex subunit DGCR8 |
| Synonyms
|
Not Available
|
| Gene Name
| DGCR8 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in double-stranded RNA binding |
| Specific Function
| Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 17 |
| Locus
| Not Available |
| SNPs
| DGCR8 |
| Gene Sequence
|
>2283 bp
ATGGAGACATGTGGGAGCCCCTCTCCTCTCCCGCGCGAGCCCGCAGGAGGAGTGGCGATG
GAGGACCGAGCTCGCCCCCTCCGTGCGCTGCCCCGTGGACAGTCTCCACCACCTCCCCTG
CAAACGTCCAGTGATGCAGAGGTAATGGACGTTGGCTCTGGTGGTGATGGACAGGCCGAA
CCCCCTGCCGAGGACCCGCTCAACTTCTACGGAGCTTCTCTTCTCTCCAAAGGATCCTCC
TCTAAGGCCCGCCTCCTCGTAGACCCGAACTGTAGTGGCCACAGCCCGCGCACAGCGCGG
CATGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGATGTAAAGATT
AGCGTGAGCTTTACGGAGAGCTGCAGGAGTGAGGACAGGAAGGTGCTGTACACGGGAGCG
GAGCGCGACGTGCGGGCAGAGTGTGGCCTGGCCCTCAGCCCTGTCATTGGGGACGTGCAT
GCTGGTCCCTTTGGCGGGAGCGTGGGGAACGGGGTAGGCGCAGGGGGTGAGAGTGCGGGT
AAGAGGGATGAGGAACATGAGCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGAT
GAGTTAGAAGATTTTACTGACAATTTGGAGCTAGATGAAGAAGGCGCAGGCGGGTTCACG
GCTAAAGCGATCGTGCAGAGAGACAGAGTGGACGAAGAGGCCTTGAATTTCTCCTACGAG
GATGATTTTGACAACGATGTTGATGCCCTTCTGGAAGAGGGCCTCTGTGCTCCCAAAAAG
AGGCGAATGGAGGAGAAATACGGAGGAGACAGCGACCACCCGTCGGATGGGGAGACAAGC
GTGCAGCCAATGATGACCAAGATTAAAACCGTTCTCAAAAGCCGTGGCCGCCCGCCGACG
GAGCCGCTGCCCGATGGCTGGATCATGACGTTCCACAATTCCGGAGTGCCCGTGTACCTG
CACCGGGAGTCGCGGGTGGTCACCTGGTCCAGGCCCTACTTCCTGGGCACGGGGAGCATC
CGGAAACACGGCCCTCCCCTGACCAGCATCCCCTGCCTGCACTACCGGAAGATGAAGGAC
AGTGAGGAGCGGGAGCGGGCCGCGGGGATAGCCCCCCCCGAGCCGGAGCTGCCCCCGGAC
GAGCCCGACCCGCTGGGCACCGACGCGGGGCCCCCGGACGAGAAGGACCCGCTGGGGGCT
GAGGCGGCACCCGGGGCCCTGGGGCAGGTGAAGGCCAAGGTGGAGGTGTGCAAGGACGAG
TCGGTCGACCTTGAGGAGTTCCGGAATTACCTGGAGAAGCGCTTTGACTTTGAGCAAGTG
ACCGTGAAGAAGTTCAGGACGTGGGCTGAGCGTCGGCAGTTCAACCGAGAAATGAAGCGA
AAACAGGCCGAGTCCGAGAGGCCCATCCTGCCCGCCAACCAGAAGCTCATCACGCTGTCT
GTGCAGGACGCGCCCACGAAGAAAGAGTTTGTCATCAACCCCAACGGGAAGTCTGAGGTG
TGCATCCTGCACGAGTACATGCAGCGCGTGCTCAAGGTCCGCCCCGTGTATAGCTTCTTT
GAGTGCGAGAACCCAAGTGAGCCTTTTGGTGCCTCGGTGACCATTGACGGTGTGACCTAT
GGATCTGGAACGGCGAGCAGCAAAAAACTGGCCAAGAACAAAGCTGCCCGCGCCACGCTG
GAGATCCTCATCCCCGACTTCGTCAAGCAGACGTCGGAGGAGAAGCCCAGAGACAGCGAG
GAGCTGGAGTATTTCAACCACATCAGTATCGAGGACTCGCGGGTCTACGAGCTGACCAGC
AAGGCCGGGCTGCTCTCCCCCTACCAGATCCTCCACGAGTGCCTTAAAAGAAACCACGGG
ATGGGAGATACCTCCATCAAGTTTGAAGTGGTTCCTGGTAAAAACCAGAAGAGCGAATAC
GTCATGGCGTGCGGCAAGCACACAGTGCGCGGCTGGTGCAAGAACAAGCGCGTTGGGAAG
CAGTTGGCGTCTCAGAAGATCCTGCAACTGCTGCACCCGCATGTCAAGAACTGGGGGTCC
TTGCTGCGCATGTATGGCCGTGAGAGCAGCAAGATGGTCAAGCAGGAGACCTCGGACAAG
AGCGTGATCGAGCTGCAGCAGTTCGCTCGCAAGAACAAGCCCAACCTGCACATCCTGAGC
AAGCTGCAGGAGGAGATGCGGCGGCTGGCGGAGGAGCGGGAGGAGACGCGCAAGAAGCCC
AAGATGTCCATCGTGGCTTCCGCACAGCCCGGCGGCGAGCCCCTGTGCACCGTGGACGTG
TGA
|
| Protein Properties |
|---|
| Number of Residues
| 760 |
| Molecular Weight
| 84383.0 |
| Theoretical pI
| 6.3 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 30-56
- 77-98
- 113-133
- 140-158
- 178-200
- 229-246
- 288-307
- 319-338
- 350-372
|
| Protein Sequence
|
>Microprocessor complex subunit DGCR8
METCGSPSPLPREPAGGVAMEDRARPLRALPRGQSPPPPLQTSSDAEVMDVGSGGDGQAE
PPAEDPLNFYGASLLSKGSSSKARLLVDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKI
SVSFTESCRSEDRKVLYTGAERDVRAECGLALSPVIGDVHAGPFGGSVGNGVGAGGESAG
KRDEEHELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFSYE
DDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPT
EPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHGPPLTSIPCLHYRKMKD
SEERERAAGIAPPEPELPPDEPDPLGTDAGPPDEKDPLGAEAAPGALGQVKAKVEVCKDE
SVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLS
VQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYSFFECENPSEPFGASVTIDGVTY
GSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPRDSEELEYFNHISIEDSRVYELTS
KAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGK
QLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQFARKNKPNLHILS
KLQEEMRRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
|
| External Links |
|---|
| GenBank ID Protein
| AAI50110.1 |
| UniProtKB/Swiss-Prot ID
| A6QR44 |
| UniProtKB/Swiss-Prot Entry Name
| DGCR8_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC150109 |
| GeneCard ID
| DGCR8 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
