| Identification |
|---|
| BMDB Protein ID
| BMDBP02005 |
| Secondary Accession Numbers
| None |
| Name
| Poly(U)-binding-splicing factor PUF60 |
| Synonyms
|
Not Available
|
| Gene Name
| PUF60 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in DNA binding |
| Specific Function
| DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 14 |
| Locus
| Not Available |
| SNPs
| PUF60 |
| Gene Sequence
|
>1593 bp
ATGGCGACGGCGACCATAGCGCTAGGGACAGACTCCATCAAGATGGAGAACGGGCAGGGC
ACAGCCGCGAAGCTGGGACTGCCTCCCTTGACGCCCGAGCAGCAGGAGGCCCTCCAGAAG
GCCAAGAAGTACGCCATGGAGCAGAGCATCAAGAGCGTGCTGGTGAAACAGACCATCGCC
CACCAGCAGCAGCAGCTTACCAACCTGCAGATGGCAGCAGTGACAATGGGCTTTGGAGAT
CCTCTCTCACCTTTGCAATCGATGGCGGCTCAGCGGCAGCGGGCACTGGCCATCATGTGC
CGGGTCTACGTGGGCTCCATCTACTACGAGCTAGGGGAGGACACCATCCGCCAGGCGTTC
GCCCCCTTTGGGCCTATCAAGAGCATCGACATGTCCTGGGACTCCGTCACCATGAAGCAC
AAGGGCTTTGCGTTTGTGGAGTATGAGGTCCCAGAAGCCGCCCAGCTGGCCTTGGAGCAG
ATGAATTCCGTGATGCTGGGAGGCAGGAACATCAAGGTGGGCAGACCCAGCAACATAGGG
CAGGCTCAGCCCATCATAGACCAGCTGGCCGAGGAGGCGCGAGCCTTCAACCGCATCTAC
GTGGCTTCCGTGCACCAGGACCTCTCGGATGATGACATCAAGAGTGTGTTTGAGGCCTTT
GGCAAGATCAAATCCTGCACGCTGGCTCGGGACCCCACGACTGGCAAACACAAGGGCTAT
GGCTTCATTGAATATGAGAAAGCCCAATCGTCCCAGGATGCTGTGTCTTCCATGAACCTT
TTTGACCTGGGGGGCCAGTACTTGCGGGTGGGCAAGGCTGTCACACCCCCTATGCCCCTG
CTTACACCGGCCACACCTGGAGGCCTCCCGCCGGCTGCTGCCGTGGCCGCAGCTGCAGCC
ACAGCCAAGATTACAGCTCAGGAAGCAGTGGCTGGAGCAGCAGTACTGGGTACCCTAGCC
ACTCCTGGACTGGTGTCCCCTGCACTGACTCTGGCCCAGCCCCTGGGGGCTCTACCCCAG
GCCGTCATGGCTGCCCAGGCCCCAGGAGTCATCACAGGTGTGACACCGGCTCGGCCTCCC
ATTCCGGTCACCATCCCCTCTGTTGGTGTGGTGAATCCCATCCTGGCCAGCCCTCCGACA
CTTGGTCTCCTGGAGCCTAAGAAGGAGAAGGAGGAGGAGGAGCTGTTTCCCGAGTCGGAG
CGGCCGGAGATGCTGAGTGAGCAGGAGCACATGAGCATCTCAGGAAGCAGCGCCCGCCAC
ATGGTGATGCAGAAGCTCCTCCGCAAGCAGGAGTCCACAGTGATGGTTCTGCGTAACATG
GTGGACCCCAAGGACATCGACGATGACCTTGAAGGGGAGGTGACTGAGGAGTGTGGCAAG
TTTGGTGCTGTCAACCGTGTCATCATCTACCAAGAGAAGCAGGGCGAGGAAGAGGACGCG
GAGATCATTGTCAAGATTTTTGTGGAGTTTTCCGTAGCCTCTGAGACTCACAAGGCCATC
CAGGACCTCAATGGGCGCTGGTTTGCTGGCCGCAAGGTGGTGGCTGAAGTGTATGACCAG
GAGCGTTTTGATAACAGTGACCTCTCTGCATGA
|
| Protein Properties |
|---|
| Number of Residues
| 530 |
| Molecular Weight
| 57087.0 |
| Theoretical pI
| 4.87 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Poly(U)-binding-splicing factor PUF60
MATATIALGTDSIKMENGQGTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIA
HQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAF
APFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIG
QAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGY
GFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAA
TAKITAQEAVAGAAVLGTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPARPP
IPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARH
MVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDA
EIIVKIFVEFSVASETHKAIQDLNGRWFAGRKVVAEVYDQERFDNSDLSA
|
| External Links |
|---|
| GenBank ID Protein
| AAI05448.1 |
| UniProtKB/Swiss-Prot ID
| Q2HJG2 |
| UniProtKB/Swiss-Prot Entry Name
| PUF60_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC105447 |
| GeneCard ID
| PUF60 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
