| Identification |
|---|
| BMDB Protein ID
| BMDBP01341 |
| Secondary Accession Numbers
| |
| Name
| Retinoid isomerohydrolase |
| Synonyms
|
Not Available
|
| Gene Name
| RPE65 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Secondary metabolites biosynthesis, transport and catabolism |
| Specific Function
| Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16096063, PubMed:19805034, PubMed:20100834). Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (By similarity). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (PubMed:15186777). |
| Pathways
|
Not Available
|
| Reactions
|
| Retinyl ester + Water → 11-cis-Retinol + Fatty Acid |
details
|
| cis-b,b-Carotene + Oxygen → Retinal |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 3 |
| Locus
| Not Available |
| SNPs
| RPE65 |
| Gene Sequence
|
>1602 bp
ATGTCCAGCCAAGTTGAACATCCAGCTGGTGGTTACAAGAAACTGTTTGAAACTGTGGAG
GAACTATCCTCACCGCTCACAGCCCATGTTACAGGCAGGATCCCCCTCTGGCTAACCGGC
AGTCTCCTTCGATGTGGGCCAGGACTCTTTGAGGTTGGATCGGAACCATTTTACCACCTG
TTTGATGGGCAAGCCCTCCTACACAAGTTTGACTTTAAAGAAGGACATGTCACATACCAC
AGAAGGTTCATCCGCACTGATGCTTACGTACGGGCAATGACTGAGAAAAGGATCGTCATA
ACAGAATTTGGCACCTGTGCTTTCCCAGATCCCTGCAAGAATATATTTTCCAGGTTTTTT
TCTTACTTCCGAGGAGTGGAGGTTACTGACAATGCCCTTGTTAATATCTACCCAGTGGGG
GAAGATTACTATGCCTGCACAGAGACCAACTTCATTACAAAGGTTAATCCTGAGACCTTG
GAAACAATTAAGCAGGTTGACCTTTGCAACTATGTCTCAGTCAATGGAGCCACTGCTCAC
CCCCACATTGAAAATGATGGGACTGTTTACAACATTGGTAATTGCTTTGGGAAAAATTTT
TCAATTGCCTACAATATTGTAAAGATCCCACCACTACAAGCAGACAAGGAAGATCCAATA
AGCAAGTCAGAGATCGTTGTACAATTCCCCTGCAGTGACCGATTCAAGCCATCTTACGTC
CATAGTTTTGGTTTGACTCCCAACTATATTGTTTTTGTGGAGACACCAGTCAAAATTAAT
CTGTTCAAGTTTCTTTCTTCATGGAGTCTTTGGGGAGCCAATTACATGGATTGTTTTGAA
TCCAATGAAACCATGGGGGTTTGGCTTCATATTGCTGACAAAAAAAGAAAAAAGTATATC
AATAATAAATACAGGACCTCTCCTTTTAACCTCTTTCATCACATCAATACCTATGAAGAC
CATGAGTTTCTGATTGTGGATCTCTGTTGCTGGAAAGGATTTGAATTTGTTTATAATTAT
TCATATTTAGCCAATTTACGTGAGAACTGGGAAGAGGTGAAAAAAAATGCCAGAAAGGCT
CCTCAGCCTGAAGTTAGGAGATACGTACTTCCTTTGAATATTGACAAGGCTGACACAGGC
AAGAATTTAGTCACACTCCCCAACACAACTGCCACTGCAATTCTGTGCAGTGACGAGACC
ATCTGGCTGGAACCTGAGGTTCTCTTTTCAGGGCCTCGCCAAGCATTTGAGTTTCCTCAA
ATCAATTACCAGAAGTATGGTGGGAAACCTTACACATATGCATATGGACTTGGCTTGAAT
CACTTTGTTCCAGACAGGCTCTGTAAGCTGAACGTCAAAACTAAAGAAACCTGGGTATGG
CAAGAGCCTGATTCATACCCCTCAGAACCTATCTTTGTTTCTCACCCAGATGCCTTGGAG
GAAGATGACGGTGTAGTTCTGAGTGTGGTGGTGAGCCCTGGGGCAGGACAAAAGCCTGCT
TATCTTCTGATTCTGAATGCCAAGGACTTGAGTGAAGTTGCCAGGGCTGAAGTGGAGATT
AACATCCCCGTCACCTTTCATGGACTGTTCAAAAAATCCTGA
|
| Protein Properties |
|---|
| Number of Residues
| 533 |
| Molecular Weight
| 60944.0 |
| Theoretical pI
| 6.41 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Retinal pigment epithelium-specific 65 kDa protein
MSSQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHL
FDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFF
SYFRGVEVTDNALVNIYPVGEDYYACTETNFITKVNPETLETIKQVDLCNYVSVNGATAH
PHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYV
HSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYI
NNKYRTSPFNLFHHINTYEDHEFLIVDLCCWKGFEFVYNYSYLANLRENWEEVKKNARKA
PQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQ
INYQKYGGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALE
EDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS
|
| External Links |
|---|
| GenBank ID Protein
| AAC37306.1 |
| UniProtKB/Swiss-Prot ID
| Q28175 |
| UniProtKB/Swiss-Prot Entry Name
| RPE65_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| L11356 |
| GeneCard ID
| RPE65 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
